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. 2012 Oct 16;7(10):e47713. doi: 10.1371/journal.pone.0047713

Figure 3. Effect of HFD on changes in the gut microbiota system of mice.

Figure 3

Taxonomy compositions: (A) phylum and (B) family levels are shown (individual samples are on the left panels and pooled samples are on the right panels). Genomic DNA was extracted from the cecal samples taken from mice maintained for 8 weeks on a LFD and HFD. Samples were analyzed for the bacterial composition by pyrosequencing of the bacterial 16S rRNA fragments (n = 5). (C) Number of Enterobacteriaceae in DHL and Bifidobacteria in BL agar plates and (D) endotoxin content of fecal microflora cultured in GAM or GAM (-glucose) containing LFD or HFD were measured. n.d., not detected, All values were indicated as the mean ± SEM (n = 7). *, p<0.05