Figure 2. Datura stramonium is a restrictive host to infection by Iris yellow spot virus (IYSV).

(A) Inoculated leaves with local lesions as a result of virus infection; (B) younger, uninoculated leaves of the same plant remain symptomless and virus-free; (C) Detection of IYSV genes in inoculated leaves of D. stramonium using reverse transcription-polymerase chain reaction. Total RNA from TSWV inoculated leaf (1) and healthy plant leaf as negative control (2) was used as a template for amplifying the genes using specific primers as described in Table 1. M: 1 kb Marker; N: IYSV nucleocapsid (N) protein gene; NSs: IYSV nonstructural (NSs) protein gene; NSm: IYSV nonstructural (NSm) protein gene; G_N/G_C: IYSV glycoprotein precursor (G_N/G_C); RdRp: IYSV RNA dependent RNA polymerase (RdRp).