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. 2012 Oct 16;7(10):e47644. doi: 10.1371/journal.pone.0047644

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© 2012 Tessadori et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A–C) Optical mapping on an explanted, contracting adult zebrafish tg(isl1BAC:GalFF; UAS:GFP) heart. Arrow indicates the sinus venosus in all panels. (A) Explanted adult zebrafish heart. (B) GFP-fluorescent cells reporting Isl1 expression are situated at the sinus venosus. (C) The activation pattern measured by di-4-ANEPPS fluorescence shows that the GFP+ myocytes are situated in the area of earliest activation. (D) Typical action potentials of freshly isolated GFP⁺ and GFP⁻ myocytes. The GFP⁻ cell was stimulated at 3 Hz. The inset displays a representative example of a GFP+ myocyte.