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. Author manuscript; available in PMC: 2012 Oct 16.
Published in final edited form as: Mol Cell. 2011 Dec 15;45(1):64–74. doi: 10.1016/j.molcel.2011.11.015

Figure 6. The dTip60 Histone Acetyltransferase Is Necessary for Acetylation of Histone H2A Lysine 5 and Activation of PARP upon Heat Shock.

Figure 6

(A) ChIP of H2AK5 acetylation at the Hsp70Ab HS locus in dTip60 knockdown cells. S2 cells RNAi-depleted of dTip60 and heat-shocked for either 0 (light blue) or 2 min (pink) are compared to LacZ RNAi cells heat-shocked for 0 (dark blue) or 2 min (red). The level of H2AK5 acetylation is normalized to ChIP values for histone H2A and plotted on the y axis. Error bars represent the SEM of three independent experiments for all three panels.

(B) ChIP of PARP in dTip60 knockdown cells. S2 cells RNAi depleted of dTip60 and heat-shocked for either 0 (light blue) or 2 min (pink) are compared to LacZ RNAi treated cells heat-shocked for 0 (dark blue) or 2 min (red).

(C) ChIP of PAR in dTip60 knockdown cells as in (B).