Fig. 7.

Incorporation of trans-fats into the free fatty acid, triglyceride, polar lipid and cholesteryl ester pools of fatty acid containing hepatic lipids after trans-fat feeding for 24 weeks or after trans-fat feeding for 16 weeks followed by crossover to control chow for a further 8 weeks. No trans-fats were detectable in any of the pools in control mice fed standard chow for the 24 week period (not shown). The polar lipid pool, mostly phosphatidylcholine, was the most trans-fat enriched pool in the liver and retained the greatest amount after crossover to control chow. The cholesteryl ester pool by comparison was mostly devoid of trans-fats after crossover to control chow, suggesting rapid turnover. The data represents the mean of analyses of 5–9 mice per group, error bars indicate standard deviation; the percent trans-fat incorporation into the fatty acids pools was significantly less in the crossover group compared to the ALIOS group for each lipid pool (P < 0.001 for all groups)