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. Author manuscript; available in PMC: 2013 Nov 25.
Published in final edited form as: Mol Cell Endocrinol. 2012 Aug 30;364(1-2):83–88. doi: 10.1016/j.mce.2012.08.012

Figure 1.

Figure 1

Estradiol effect on hTERT mRNA expression in cultured human PBMCs. Cells were cultured in phenol red-free RPMI 1640 with 10% charcoal-stripped serum (basal conditions) or with added anti-CD3/CD28 microspheres (left panel) or PHA (right panel) to drive T cell activation. Estradiol was added to cultures in concentrations ranging from 0.1 to 1000 nM. Relative expression of hTERT mRNA (compared to unstimulated cells) was determined using simultaneous quantitation of GAPDH mRNA and calculation by the Ct method. Shaded areas of each panel show mean ± SEM for hTERT induction by anti CD3/CD28 or PHA without added estradiol.