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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Acta Neuropathol. 2012 Sep 2;124(5):665–680. doi: 10.1007/s00401-012-1040-2

Fig. 1. Quantitative assessment of myenteric neurons.

Fig. 1

Absolute numbers (neurons/mm2) and relative proportions of myenteric neuron phenotypes were assessed using double-label immunofluorescence. a. Myenteric ganglion from colon demonstrating double-label immunofluorescence for NOS (green) and HuC/D (red). Double-labeled NOS-positive neurons are yellow/orange. NOS-negative neurons (HuC/D only) are red. NOS-positive processes are green (arrows). b. Myenteric ganglion from stomach demonstrating double-label immunofluorescence for VIP (green) and HuC/D (red). Double-labeled VIP-positive neurons are yellow/orange. VIP-negative neurons (HuC/D only) are red. VIP-positive processes are green (arrows). c. Myenteric ganglion from stomach demonstrating double-label immunofluorescence for TH (green) and HuC/D (red). Double-labeled TH-positive neurons are yellow/orange. TH-negative neurons (HuC/D only) are red. TH-positive processes are green. Dotted lines outline ganglion area. LM, longitudinal muscle layer; CM, circular muscle layer. Scale bar = 100 μm