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. Author manuscript; available in PMC: 2013 Oct 15.
Published in final edited form as: Cancer Res. 2012 Aug 22;72(20):5219–5229. doi: 10.1158/0008-5472.CAN-12-1463

Figure 4. TNF-α induces netrin-1 production via NF-κB activation.

Figure 4

A TNF-α-induced netrin-1 decreases in YAMC cells at 24 hrs when pre-treated with Bay11-7085; NF-κB activation decreases using antibody to nuclear localization signal of NF-κB/p65. B Similarly, Bay11-7085 blocks netrin-1 in YAMC cells exposed to E. faecalis-infected macrophages using the dual-chamber co-culture system. C Colon macrophages from Il10−/− mice colonized with E. faecalis for 9 months (bottom, inset, green arrows, 40X) stain intensely for nuclear localization sequence of NF-κB/p65 (20X); epithelial cells also stain strongly (bottom, inset, red arrows); in contrast, weak or no staining is seen for sham-treated mice (top).