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. Author manuscript; available in PMC: 2013 Oct 15.
Published in final edited form as: Cancer Res. 2012 Aug 22;72(20):5219–5229. doi: 10.1158/0008-5472.CAN-12-1463

Figure 5. TNF-α increases Tnfrsf1b receptors.

Figure 5

A Expression of Tnfrsf1b receptors increases in YAMC cells at 100 ng/ml TNF-α at 24, 48, and 72 hrs whereas Tnfrsf1a receptors are slightly reduced.B At 48 hrs, TNF-α concentrations ≤100 ng/ml increase Tnfrsf1b in YAMC cells in a dose–dependent fashion. No significant increases are seen for Tnfrsf1a.C, Tnfrsf1b production increases for YAMC cells co-cultured with E. faecalis-infected macrophages at 24, 48, and 72 hrs. D, At 48 hrs, Tnfrsf1b production increases in YAMC cells co-cultured with E. faecalis-infected macrophages in a dose-dependent manner.E, Heat-inactivated TNF-α and anti-TNF-α antibody block TNF-α induction of Tnfrsf1b compared to control. F Immmunohistochemical staining shows increased Tnfrsf1b in colonic epithelial cells from Il10−/− mice colonized with E. faecalis for 9 months compared to sham; no change is seen for Tnfrsf1a (20X).