Table 1.
Summary of substrate effects on seedling secretion of hydrogen peroxide into agarose media
| Agarose medium amendment | Seed treatment prior to germination | Percentage of seedlings showing H2O2 secretion zonesa | Average intensity of H2O2 zonesb |
|---|---|---|---|
| Nonec | Disinfection | 23.08 | + |
| None | None | 100 | +++ |
| Yeast Extract (0.01 %) | None | 100 | +++ |
| Yeast Extract (0.1 %) | None | 54 | ++ |
| Alanine (0.01 %) | None | 96.3 | +++ |
| Alanine (0.1 %) | None | 21.05 | + |
| Glycine (0.01 %) | None | 88 | +++ |
| Glycine (0.1 %) | None | 40 | + |
| Inactivated Cellulase (0.1 %) | Disinfection | 100 | +++ |
| Ammonium Nitrate (0.01 %) | None | 86.2 | +++ |
| Ammonium Nitrate (0.1 %) | None | 78.13 | +++ |
| Potassium Nitrate (0.01 %) | None | 76.67 | +++ |
| Potassium Nitrate (0.1 %) | None | 86.67 | +++ |
aAverage of 100 seedlings assessed
b+ = low intensity or lighter zones, +++ is higher intensity or darker zones, and ++ are intermediate intensity zones
cControl from cellulase enzyme amendment experiment