Fig. 4.

Effects of short-term in vivo hormone treatments on levels of Aβ clearance factors. A, Representative agarose gel of RT-PCR products shows the relative levels of IDE, ACE, ECE2, and TTR mRNA in sham OVX (Sham), vehicle-treated OVX (OVX), and OVX rats after short-term treatment with E₂ (OVX+E₂) or P₄ (OVX+P₄). B–E, Quantitative real-time PCR data show the mean (±sem) expression levels compared with the Sham control group for IDE, ACE, ECE2, and TTR mRNA, respectively. All data are normalized with corresponding β-actin values. Statistical significance is based on analysis of pooled raw data using the Tukey HSD. *, P < 0.01 relative to the vehicle-treated Sham group. •, P < 0.01 relative to the OVX group.