Fig. 3.

The expression of some genes is Fe-responsive, TH-responsive, or neither in the neonatal hippocampus and cerebral cortex. Hippocampi or cerebral cortices were harvested from P10 male pups (n = 8–10), total RNA was extracted, and cDNA was synthesized. Quantitative real-time PCR was performed for several Fe-responsive, TH-responsive, or negative control genes. A, Transferrin receptor 1 (TfR1). B, Divalent metal transporter 1 (Dmt1). C, Angiotensinogen (Agt). D, Myelin-associated oligodendrocyte basic protein (Mobp). E, Neurogranin (Rc3). F, Dihydropyrimidinase-like 3 (Dpysl3). G, Early growth response factor 1 (Egr1). H, Glyceraldehyde 3-phosphate dehydrogenase (Gapdh). Relative mRNA levels are calculated relative to an internal control cDNA sample. Groups not sharing a common superscript are significantly different by one-way ANOVA and Tukey's or Scheffé's multiple comparison test (P < 0.05). The 3-ppm PTU group served as a positive hypothyroid control. Asterisks indicate statistical difference between 3 ppm PTU and control.