Table 2.
Summary of Annexin positive cells in wtp-53 lymphoma cell lines exposed to HDM2-SMIs
| Cell line | Time (h) | Conc. (μM) | Nutlin-3 | MI-219 | ANOVA pa | Post-hoc pb |
|---|---|---|---|---|---|---|
| WSU-FSCCL |
12 h |
0 |
5.3 ± 0.7 |
5.3 ± 0.7 |
p = <0.001 |
-- |
| 2.5 |
7.0 ± 2.5 |
13.7 ± 1.3 |
p = <0.001 |
|||
| 5.0 |
8.8 ± 2.0 |
23.8 ± 2.8 |
p = <0.001 |
|||
| 10.0 |
10.6 ± 1.7 |
36.1 ± 2.3 |
p = <0.001 |
|||
| 24 h |
0 |
6.2 ± 0.7 |
6.0 ± 0.7 |
p = <0.0001 |
-- |
|
| 2.5 |
9.6 ± 1.6 |
27.6 ± 8.2 |
NS |
|||
| 5.0 |
12.3 ± 2.4 |
43.0 ± 18.6 |
p = <0.01 |
|||
| 10.0 |
17.3 ± 4.9 |
69.7 ± 6.6 |
p = <0.001 |
|||
| 48 h |
0 |
6.1 ± 1.2 |
6.1 ± 1.2 |
p = <0.0001 |
-- |
|
| 2.5 |
10.1 ± 0.7 |
19.5 ± 1.0 |
NS |
|||
| 5.0 |
12.6 ± 1.9 |
35.6 ± 7.6 |
p = <0.001 |
|||
| 10.0 |
16.5 ± 2.6 |
74.5 ± 4.9 |
p = <0.001 |
|||
| KM-H2 | 12 h |
0 |
7.9 ± 1.2 |
7.9 ± 1.2 |
p = 0.026 |
-- |
| 2.5 |
11.2 ± 1.6 |
12.5 ± 1.3 |
NS |
|||
| 5.0 |
11.8 ± 1.5 |
13.5 ± 0.6 |
NS |
|||
| 10.0 |
13.6 ± 2.4 |
14.8 ± 2.9 |
NS |
|||
| 24 h |
0 |
8.9 ± 1.7 |
8.9 ± 1.7 |
p = 0.026 |
-- |
|
| 2.5 |
11.2 ± 3.0 |
14.2 ± 2.5 |
NS |
|||
| 5.0 |
14.5 ± 3.0 |
19.9 ± 3.5 |
NS |
|||
| 10.0 |
20.1 ± 3.8 |
27.9 ± 4.8 |
p = 0.05 |
|||
| 48 h | 0 |
2.8 ± 2.5 |
12.8 ± 2.5 |
NS | ||
| 2.5 |
15.9 ± 6.5 |
19.7 ± 9.4 |
||||
| 5.0 |
18.9 ± 8.9 |
23.1 ± 10 |
||||
| 10.0 | 21.1 ± 9.7 | 32.4 ± 9.2 |
a: ANOVA for overall significance between all groups. b: Tukey’s post hoc comparisons between pairs (Nutlin-3 versus MI-219 at equivalent concentrations).