Table 1.

Equilibrium constants, rate constants and allosteric parameters determined by global fitting of experimental data using Model 2

Parameter	Value or expression	Parameter	Value or expression
k12		pK	7.9
k21		α	0.06 s−1
k23		β	229 s−1
k32		f1	151.6
p1	10 RT	f2	8.4
p2	5 RT	fH	8.46
s1	−5 RT
d1	0.2

ν = kT/h, where k, T and h are the Boltzmann constant, temperature and the Planck constant, respectively. The p_i, S_i and d_i parameters represent the barrier high, well depth and fractional electrical distance from extracellular media, respectively (see Fig. 6D). These values or expressions were used in the simulations shown in Figs 6 and 7.