Figure 7. The open circle symbol for LF-SED was essentially overlapped by the filled symbols for HF-RUN, HF-RUN + Apocynin, and HF-SED + Apocynin, which did not differ from one another. In contrast, HF-SED was significantly less.

A, apocynin restored endothelium-dependent vasodilatation in arterioles from HF-SED mice, although apocynin did not change it in HF-RUN mice relative to HF-RUN only. Protein expression of NAD(P)H oxidase subunits, p22^phox (B), p47^phox (C) and p67^phox (D) had no significant differences among 3 groups of LF-SED, HF-SED and HF-RUN. Protein expression of NAD(P)H oxidase subunits, gp91^phox (E) was significantly higher in HF-SED compared with LF-SED mice. Voluntary wheel running opposed the development of enhanced gp91^phox protein of coronary arterioles from high fat diet fed mice. Data were expressed as means ± SEM. *P < 0.05 vs. LF-SED, #P < 0.05 vs. HF-SED.