Table 5.
Primers used for PCR amplifications of candidate genes in P. vulgaris in the Core and wild collections
| Gene | Primer Name | F/R | Sequence (5′-3′) | Ta (°C) | Source | Sequence* |
|---|---|---|---|---|---|---|
|
Asr1 (region 1) |
ADOC01_01_Pv_12 |
F |
GAGGAGACTAAGCCCATAGA |
62 |
IRD |
TC2798 |
| ASR_TC2798_RV** |
R |
TGGACAGAAGCCATTCACTCCCAA |
CIAT |
|||
|
Asr2 (region 2) |
ASR_TC2798_FW2 |
F |
AAGCACCACAAGCATCTTGAGCAC |
53 |
CIAT |
|
| ** |
R |
** |
** |
|||
|
Asr2 |
ADOC01_03_Pv_05 |
F |
CCACCACCACAAAGAGGA |
60 |
IRD |
CA910244 |
| ADOC01_03_Pv_06 | R | CAAACATTCTTCAAACTTGCTCAGA | IRD |
*Accession ID of the original EST sequences used to design the primers. TC accessions are from Gene Index (compbio.dfci.harvard.edu/tgi/).**The reverse primer ASR_TC2798_RV was also used in this case.