Figure 4.

Nanog Null EpiSC Are Reverted to Naive Pluripotency by Esrrb Expression

(A) Nanog^−/− EpiSCs carrying doxycycline-inducible Nanog or Esrrb transgenes were plated in FCS/LIF/GMEMβ with doxycycline for the indicated times. After 7 days, plates were stained for AP.

(B) Scoring of the AP colonies obtained from the experiment described in (A). Error bars: standard deviation (n = 3).

(C) mRNA expression in uninduced EpiΔN-iEsrrb and the reverted Epi-iPSΔN-iEsrrb ESC-like colonies obtained by induction of Esrrb and expansion in the absence of selection and doxycycline. Error bars: standard deviation of gene expression in two independent experiments.

(D) Brightfield (top panels) and fluorescence (bottom panels) images of ESΔN-iEsrrb, EpiΔN-iEsrrb, and Epi-iPSΔN-iEsrrb cells.

(E) AP-positive colonies of Epi-iPSΔN-iEsrrb cells grown in N2B27 supplemented with BMP/LIF (top) or 2i/LIF (bottom).

(F) Chimeric mouse obtained from a blastocyst injection with Epi-iPSΔN-iEsrrb cells.

See also Table S3.