Figure 4.

Induction of WIN3.7 mRNA by MeJA. Clone 245 plants (6- to 8-week-old cuttings) were sprayed with an aqueous solution containing either 0.1% Triton X-100 (Control) or 0.1% Triton X-100 containing 100 mm MeJA until the leaves were completely wetted. At the indicated times after spraying, the second and fourth fully expanded leaves were harvested. Total RNA was extracted, subjected to RNA-blot hybridization analysis, and the amount of hybridizing radioactivity was quantitated using a phosphor imager. Time points represent the hours after the beginning of the treatments.