FIG. 6.

Effect of Trolox and hydrogen peroxide (H₂O₂) on hormone-induced calcium transients in human skin fibroblasts. (A) Cell incubation protocol for analyzing the effects of Trolox and hydrogen peroxide (H₂O₂) on hormone-stimulated cytosolic Ca²⁺ signals. In this panel, HT indicates HEPES-Tris solution, M199 indicates culture medium, and peroxide indicates H₂O₂. (B) Effect of H₂O₂ on bradykinin-induced cytosolic Ca²⁺ transients. (C) Effect of Trolox on bradykinin-induced cytosolic Ca²⁺ transients in the absence and presence of H₂O₂. (D) Average amplitude of the Ca²⁺ transients for the conditions in panel B and C. (E) Average rate of decay of the Ca²⁺ transients under the conditions in panel B and C, as determined by fitting a mono-exponential function (see Supplementary Materials and Methods). A larger value indicates a slower rate of decay. In panels D and E, significant differences with the indicated conditions (a, b, c) are marked by *(p<0.05), **(p<0.01), or ***(p<0.001). Numerals (N) represent the number of individual cells analyzed in at least three independent experiments. (To see this illustration in color the reader is referred to the web version of this article at www.liebertpub.com/ars).