Table 2.

P values from comparisons (average survival of females, average survival of males, total egg production, total progeny production, and average progeny development time) of treatments within lines of the same type and comparisons of line types for each treatment.

Overall effects	Lines/treatments	Comparisons	P value of female life span	P value of male life span	P value of egg production	P value of progeny production	P value of progeny development time
Lines			0.4444	0.5330	0.0215	0.0961	0.1457
Treatments			<0.0001	0.0003	0.0039	0.0005	0.0051
Lines* treatments			0.1027	0.1603	0.0280	0.1535	0.1790
	Lines
	No perturbation control (CN)	Treatmenta	0.1969	0.6687	0.4517	0.0022	0.8001
	Control punctured (CP)	Treatmenta	<0.0001	0.0027	0.3270	0.1776	0.1773
	Selected (S)	Treatmenta	0.4262	0.0019	0.0001	0.0354	0.0012
	Treatments
	AS	Linesb	0.6967	0.1663	0.6729	0.0462
	H2O	Linesb	0.2074	0.2502	0.1727	0.0288
	NON	Linesb	0.2717	0.8843	<0.0001	0.9560
	Ac	Linesb					0.0088
	Bc	Linesb					0.8619
	Cc	Linesb					0.3675
	Dc	Linesb					0.4857
	Ec	Linesb					0.0098

^aTreatments: punctured with autoclaved spores (AS), punctured with sterile water (H₂O), and no treatment (NON).

^bLine types: selected (S), punctured with H₂O each generation (CP), and no perturbation each generation (CN).

^cA–E were F ₁ matings for the progeny development time assay (see Table 1).