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Knockdown of luciferase reporter gene in mHypoE N41 cells by CD.siRNA complexes. Cells were incubated with luciferase reporter plasmid (1 μg/well). Two hours later, after washing and changing media, luciferase siRNA (50 nM), either uncomplexed or complexed to CD, was added. After 24 h, reduction in gene expression was measured by luciferase assay. Data (n = 3) are expressed as the mean ± SEM; *p < 0.05 relative to untreated controls.
