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Knockdown of endogenous GAPDH in (a) mHypoE N41 cells and (b) primary rat (E18) hippocampal cells by CD.siRNA complexes. Cells were incubated GAPDH siRNA (100 nM), either uncomplexed or complexed to CD, for 24 h. Total RNA was extracted and reverse transcribed to cDNA for qRT-PCR, to determine the reduction in GAPDH mRNA expression. Data (n = 3) are expressed as the mean ± SEM; *p < 0.05 relative to untreated controls.
