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. 2012 Aug 25;3(10):782–792. doi: 10.1021/cn300100u

Figure 2.

Figure 2

Metabolic differentiation between freshly isolated and cultured neurons of A. californica. (a) PCA score plots of the CE-ESI-MS data uncovered differences in metabolite abundances between the freshly isolated B1 and B2 neurons. (b) In contrast, these neurons possessed indistinguishable chemistries after cell culture. Respective loading plots are shown in Figure S2. Metabolic differences were (c) clear between the freshly isolated and cultured B1 cells and (d) minor between the freshly isolated and cultured B2 neurons. These results indicate that despite metabolic dissimilarities in the freshly isolated state, B1 and B2 neuron chemistries became similar upon culturing. Key: B11–7 = freshly isolated B1; B21–7 = freshly isolated B2; cB11–5 = cultured B1; and cB21–6 = cultured B2 neuron extracts. Technical replicate measurements are included.