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Native two-dimensional SDS-PAGE and autoradiography. Extracts from maize seedling roots treated anaerobically for 70 h and labeled with 35S. A, Coomassie blue-stained native two-dimensional SDS-PAGE gel. ANP45A (a) and ANP45B (b) spots, indicated by arrows, were excised from similar gels and subjected to N-terminal sequencing. B, Autoradiograph of the gel shown in A.
