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. 1998 Dec;118(4):1285–1293. doi: 10.1104/pp.118.4.1285

Figure 5.

Figure 5

Two-dimensional SDS-PAGE and western-blot analysis of in vivo-labeled maize root proteins. Maize root proteins, labeled in vivo either under aerobic (A and B) or anaerobic treatment (C–H), were separated on two-dimensional SDS-PAGE gels and electroblotted onto nitrocellulose filters. This blot was first subjected to western-blot analysis using enolase antibody, and then was subjected to autoradiography. The bottom panels are autoradiograms representing aerobic control (B) and plants anaerobically treated for 15 h (D), 45 h (F), and 70 h (H). The top panels (A, C, E, and G) are the western blots used for the autoradiograms B, D, F, and H, respectively. The proteins recognized by the antibody that corresponds to ANP45A and ANP45B are marked with long arrowheads. The shorter arrowheads in A and B point to cross-reacting proteins that appear to be synthesized under aerobic conditions but not during anoxia. Size markers (MW) are shown on the left (in kilodaltons).