Two-dimensional SDS-PAGE and western-blot analysis
of in vivo-labeled maize root proteins. Maize root proteins, labeled in
vivo either under aerobic (A and B) or anaerobic treatment (C–H), were
separated on two-dimensional SDS-PAGE gels and electroblotted onto
nitrocellulose filters. This blot was first subjected to western-blot
analysis using enolase antibody, and then was subjected to
autoradiography. The bottom panels are autoradiograms representing
aerobic control (B) and plants anaerobically treated for 15 h (D),
45 h (F), and 70 h (H). The top panels (A, C, E, and G) are
the western blots used for the autoradiograms B, D, F, and H,
respectively. The proteins recognized by the antibody that corresponds
to ANP45A and ANP45B are marked with long arrowheads. The shorter
arrowheads in A and B point to cross-reacting proteins that appear to
be synthesized under aerobic conditions but not during anoxia. Size
markers (MW) are shown on the left (in kilodaltons).