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. Author manuscript; available in PMC: 2013 Sep 27.
Published in final edited form as: J Med Chem. 2012 Sep 18;55(18):7998–8006. doi: 10.1021/jm300804e

Figure 2.

Figure 2

FRET assays demonstrating inhibition of PA oligomerization. The dye-conjugated, nicked PA proteins nPA83 N645C*488 and nPA83 N645C*594 were added to a 96-well plate at 250 nM each. Inhibitors were added to a concentration of 100 μM to all wells except the control and oligomerization was driven by the addition of LF at a final concentration of 50 nM. Fluorescence at 610 nm was monitored in wells containing nPA83 and tested compounds in the presence of 50 nM LF. Plotted fluorescence is the difference in fluorescence of wells containing identical mixtures with and without inhibitors.