Skip to main content
. Author manuscript; available in PMC: 2013 Sep 27.
Published in final edited form as: J Med Chem. 2012 Sep 18;55(18):7998–8006. doi: 10.1021/jm300804e

Figure 4.

Figure 4

Assessment of PA binding and oligomerization to cells, and in vitro furin activity. (A) CHO C4 cells were treated with PA or PA + LF for 1 h in the presence or absence of 100 μM inhibitor and the cells were lysed and analyzed by Western blotting for PA species. (B, C) Purified PA (B) or TGFα-PE38 (C) were mixed with furin at a 100:1 molar ratio and incubated for 1 h with the indicated concentrations of inhibitors. Wells labeled ND contained no drug, and wells labeled NT were not treated with toxins in (A) or not treated with furin in (B and C).