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Confocal optical sections of GFP-expressing mossy fiber axons in the dentate inner molecular layer from PTEN KO cells are shown in red. Double-immunostaining for the presynaptic granule cell terminal marker ZnT-3 is shown in cyan. White arrows denote ZnT-3 immunoreactive, GFP-expressing axon varicosities (presumptive PTEN KO cell axon terminals), while blue arrowheads denote ZnT-3 immunoreactive, GFP negative puncta (presumptive wildtype granule cell terminals). Scale bars= 5 μm.
