Table 1. Physiological, yield attributes and nutrient analysis of PDH45 transgenic T1 rice (Oryza sativa L, CV: IR64) plants.
Parameter | H2O grown wild type | H2O grown vector control | H2O grown antisense | Progeny | Salt grown PDH45 sense transgenics (200 mM NaCl) |
||||
---|---|---|---|---|---|---|---|---|---|
Line 1 | Line 2 | Line 3 | Line 4 | Line 5 | |||||
Segregation ratio |
0 |
0 |
0 |
T1 |
3:1 [163] |
2.91:1 [158] |
3.1:1 [149] |
3.0:1 [150] |
3.2:1 [147] |
Seedlings survival (%) |
97 ± 3.9a |
94 ± 3.7a |
96 ± 4.1a |
T1 |
97 ± 4.2a |
98 ± 4.1a |
94 ± 3.8a |
97 ± 4.1a |
96 ± 3.7a |
Days for flowering |
90 ± 2.6a |
90 ± 2.8a |
90 ± 2.8a |
T1 |
90 ± 2.5a |
90 ± 2.6a |
90 ± 2.1a |
90 ± 2.7a |
90 ± 2.8a |
Plant height (cm) |
69 ± 3.1b |
65 ± 3.0b |
63 ± 3.7b |
T1 |
76 ± 3.8a |
76 ± 3.9a |
79 ± 3.5a |
78 ± 3.0a |
79 ± 2.9a |
No. of tillers/plant |
25 ± 1.0c |
25 ± 0.9c |
27 ± 1.2c |
T1 |
33 ± 1.1b |
33 ± 1.2b |
37 ± 1.0a |
32 ± 0.9b |
34 ± 1.1b |
No. of panicle/plant |
21 ± 0.7b |
20 ± 0.8b |
23 ± 0.7b |
T1 |
30 ± 1.0a |
29 ± 1.0a |
35 ± 1.2a |
30 ± 1.1a |
32 ± 1.0a |
No. of filled grain/panicle |
75 ± 3.2b |
74 ± 3.0b |
69 ± 2.5b |
T1 |
105 ± 4.1a |
108 ± 4.3a |
102 ± 4.9a |
101 ± 4.5a |
108 ± 4.7a |
No. of chaffy grains/panicle |
11 ± 0.33a |
10 ± 0.31a |
12 ± 0.29a |
T1 |
06 ± 0.21b |
05 ± 0.20b |
04 ± 0.11b |
06 ± 0.17b |
05 ± 0.15b |
Straw dry weight (g) |
55 ± 2.1b |
54 ± 2.0b |
50 ± 0.95b |
T1 |
68 ± 2.5a |
69 ± 2.1a |
72 ± 2.6a |
74 ± 2.5a |
74 ± 2.2a |
100 grain weight (g) |
2.83 ± 0.10a |
2.82 ± 0.11a |
2.9 ± 0.10a |
T1 |
3.3 ± 0.12a |
3.4 ± 0.10a |
3.5 ± 0.11a |
3.5 ± 0.12a |
3.7 ± 0.12a |
Root length (cm) |
21 ± 0.98a |
20 ± 0.87a |
20 ± 0.99a |
T1 |
22 ± 1.0a |
23 ± 1.2a |
21 ± 1.1a |
22 ± 1.0a |
24 ± 1.1a |
Root dry weight (g) |
1.79 ± 0.06a |
1.69 ± 0.07a |
1.64 ± 0.06a |
T1 |
3.7 ± 0.15a |
3.6 ± 0.15a |
3.9 ± 0.16a |
3.6 ± 0.15a |
3.8 ± 0.18a |
Total protein (mg/g FW) |
13.08 ± 0.51b |
13.17 ± 0.55b |
14.56 ± 0.4b |
T1 |
20.25 ± 0.87a |
21.28 ± 0.85a |
20.61 ± 0.88a |
22.23 ± 0.95a |
21.13 ± 0.91a |
Chlorophyll (mg/g FW) |
8.18 ± 0.39c |
9.07 ± 0.41b |
9.10 ± 0.40b |
T1 |
9.73 ± 0.42a |
9.75 ± 0.38a |
9.84 ± 0.35a |
9.72 ± 0.39a |
9.71 ± 0.37a |
Nitrogen (%) |
0.329 ± 0.012b |
0.347 ± 0.015b |
0.327 ± 0.011b |
T1 |
0.415 ± 0.015a |
0.439 ± 0.012a |
0.442 ± 0.014a |
0.427 ± 0.013a |
0.457 ± 0.016a |
Phosphorus (%) |
0.353 ± 0.010b |
0.337 ± 0.013b |
0.353 ± 0.012b |
T1 |
0.391 ± 0.012a |
0.384 ± 0.011a |
0.373 ± 0.012a |
0.381 ± 0.010a |
0.382 ± 0.012a |
Potassium (%) |
0.165 ± 0.005a |
0.163 ± 0.006a |
0.163 ± 0.005a |
T1 |
0.179 ± 0.005a |
0.172 ± 0.004a |
0.173 ± 0.005a |
0.172 ± 0.006a |
0.175 ± 0.005a |
Sodium (%) |
0.001 ± 0.0001a |
0.001 ± 0.0001a |
0.001 ± 0.0001a |
T1 |
0.001 ± 0.0001a |
0.001 ± 0.0001a |
0.001 ± 0.0001a |
0.001 ± 0.0001a |
0.001 ± 0.0001a |
Data followed by the same letter in the same row are significantly not different at p < 0.05 as determined by Duncan’s multiple range test (n = 3). Three plants from each lines were analyzed to study the effect of salinity on various growth, nutrient analysis and yield parameters. |