Figure 1. GmMYB62 interacts with all soybean SGF14s. (A) Yeast two-hybrid assay showing interaction between the GmMYB62 and all 16 SGF14 proteins in soybean. Yeast cells were co-transformed with combination of DNA-binding domain (BK, bait) and activation domain (AD, prey) fused constructs as indicated. SD/-Leu/-Trp and SD/-Ade/-His/-Leu/-Trp correspond to dropout medium lacking Trp and Leu and Ade, Trp, Leu and His, respectively. The co-transformed yeast cultures after 100X dilution were spotted on SD/-Leu/-Trp and SD/-Ade/-His/-Leu/-Trp plate and incubated at 30°C for 5 d. Positive interactions result in yeast growth on the SD/-Ade/-His/-Leu/-Trp plate. (B) BiFC assay to demonstrate the interaction between GmMYB62 and SGF14l. Nicotiana benthamiana leaves co-transformed with constructs of GmMYB62 or SGF14l fused to N-terminal (YN) and C-terminal (YC) half of YFP, respectively (as indicated) were imaged 48 h after infiltration, using confocal microscope. (i) GmMYB62-YN+SGF14l-YC; (ii) SGF14l-YN+GmMYB62-YC. Images are shown as YFP, bright field only and merged YFP and bright-field of epidermal N. benthamiana leaf cells. Scale bars indicate 40 µm.