Fig. 3.
Southern blot analysis of LHV-4 genomic structure with only ICP0 and ICP4 probes. LHV-4 viral DNA was digested with BamHI and EcoRI. After processing, the blot was hybridized with Dig-labeled ICP0 DNA probe. The membrane was stripped and re-probed using Dig-labeled ICP4 DNA probe. The digested LHV-4 DNA was electrophoresed through a 0.7% agarose gel run at 2.5V/cm for 24 h. Lanes V: virion, N: nucleocapsid DNA, M: DIG-labeled DNA Molecular Weight Marker VII (Roche Applied Science).
