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. Author manuscript; available in PMC: 2013 Nov 10.
Published in final edited form as: Virology. 2012 Aug 22;433(1):183–191. doi: 10.1016/j.virol.2012.08.002

Fig. 4.

Fig. 4

Southern blot analysis of LHV-4 genomic structure with multiple probes. LHV-4 viral DNA was digested with BamHI and EcoRI. After processing, the blots were hybridized with Dig-labeled ICP0 DNA probe. The membrane was stripped and reprobed using Dig-labeled ICP4, US1, UL1, and UL56 DNA probes. Digested LHV-4 DNA was electrophoresed through a 1.0% agarose gel at 2.5 V/cm for 16 h. Lanes V: virion DNA, N: nucleocapsid DNA, M: DIG-labeled DNA Molecular Weight Marker VII (Roche Applied Science). The marker sizes are indicated in bp. Note: the UL1 probe was hybridized to a different blot obtained under the same conditions as the others. Open arrow: EcoRI fragments at either 25kb or 31 kb. Solid arrow: EcoRI fragment at either 4kb or 10kb. Arrow: EcoRI fragments at 25 kb and 31kb.