Table 3. Percent decrease of the marker pigment concentrations (mean ± standard deviation of triplicate microcosms, and the probability) treated with 1.6 mg L−1 H2O2, as compared to the control without H2O2 addition, in microcosms with unfiltered and 5-μm filtered seawater.
Fucoxanthin | Chl b | Peridinin | Zeaxanthin | |
Microcosms with unfiltered seawater | ||||
0 hr | 2±3% (0.248a) | 3±4% (0.452) | N.A. | 15±5% (0.023) |
24 hr | 55±2% (0.006) | 53±2% (0.007) | 34±5% (0.010) | 17±12% (0.136) |
48 hr | 44±1% (0.000) | 58±1% (0.001) | 18±18% (0.292) | N.A. |
72 hr | −104 b±3% (0.003) | 8±9% (0.018) | N.A. | 48±8% 0.012) |
Microcosms with 5 μm filtered seawater | ||||
0 hr | 4±4% (0.224) | 0±6% (0.939) | 22±15% (0.289) | 0±9% (0.978) |
24 hr | 60±7% (0.002) | 54±6% (0.003) | N.A. | N.A. |
48 hr | 57±5% (0.002) | 67±2% (0.003) | N.A. | N.A. |
72 hr | −66 b±5% (0.000) | 17±4% (0.018) | N.A. | 55±4% (0.010) |
The values in parenthesis are probabilities of the two sample t-tests; p<0.05 suggests the percent change is statistically significant. b A negative percentage value indicates pigment concentrations were greater in treatment microcosms than the controls. N.A. indicates pigment quantification might not be accurate as at least one of the triplicates went below detection limit.