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Two pairs of degenerate primers, COM1_F&COM1_R (Comamonas-type), and PSE1_F&PSE1_R (Pseudomonas-type) (Table 1) and different DNA templates: 1, 2 - 13C-DNA; 3, 4 - 12C-DNA; 5– blank control were used. M - DNA molecular weight Ladder.
