Figure 3. Activation of RhoA in MA104 cells infected with rotavirus.

MA104 cells were infected with the reassortant rotavirus strain DxRRV at a m.o.i. of 10; at the indicated times, cells were lysed and analyzed by Western blotting to detect the presence of activated RhoA. MWM, molecular weight markers with protein weights indicated; RhoA, recombinant 6xHis-RhoA protein (20 ng) used as control; C− and C+, mock cells treated with GDP and GTPγS used as negative and positive controls respectively, for RhoA activation; h.p.i, hours post infection at which infected cells were processed; Mock, mock infected cell lysate used as negative control.