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. 2012 Oct 17;7(10):e47612. doi: 10.1371/journal.pone.0047612

Figure 4. Prevention of RhoA activation in MA104 cells infected with rotavirus previously treated with anti-VP5* non-neutralizing mAbs.

Figure 4

MA104 cells were infected with the reassortant rotavirus strain DxRRV previously treated (1 h at 37°C) or not with an excess of the anti-VP5* non-neutralizing mAb HS2; at the indicated times, cells were lysed and analyzed by Western blotting to detect the presence of activated RhoA. MWM, molecular weight markers with protein weights indicated; RhoA, recombinant 6xHis-RhoA protein (20 ng) used as control; C− and C+, mock cells treated with GDP and GTPγS used as negative and positive controls respectively, for RhoA activation; h.p.i, hours post infection at which infected cells were processed, lanes corresponding to the cell infected with the anti-VP5* mAb treated virus are indicated; Mock, mock infected cell lysate used as negative control.