Figure 8. Summary of the actin perturbations in rotavirus infected cells.

A: Early interaction of rotaviruses with α2β1 integrin trigger the phosphorilation of RhoA, which would lead the formation of actin stress fibers. The participation of Rock-1 and Cofilin in this process remains to be determined. The distribution of actin filaments is not affected at this time. B: The no interaction of VP5* with α2β1 integrin prevents the phosphorilation of RhoA and the appearance of actin stress fibers. C: At 7 h.p.i. actin filaments are disorganized and actin stress fibers are observed, although phosphorolated RhoA is no longer detected. D: Silencing of rotavirus NSP4 protein or treatment with the Ca²⁺ chelator BAPTA, results in reorganization of the actin filaments. However, the results suggest that neither the activity of NSP4 nor increased intracytoplasmatic Ca²⁺ levels have a role on the formation of actin stress fibers. RV: Rotavirus. SR: Sialic Receptor.