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. 2012 Oct 17;7(10):e47623. doi: 10.1371/journal.pone.0047623

Figure 1. Design of transgenic rescue constructs.

Figure 1

(A) Transgene mini cassette construct where transgene expression was driven by the putative endogenous promoter fragment of Ppp1cc gene. A genomic fragment of ∼2.6 kb immediately upstream of the transcription start site was employed as the endogenous promoter to drive the expression of full length mouse Ppp1cc2 cDNA (with intact 5′UTR and 3′UTR). (B) Testis specific human Pgk2 promoter driven transgene cassette. In this mini gene the mouse Ppp1cc2 coding sequence used lacked the 5′ and 3′UTRs. In both constructs the SV40 PolyA signal sequence was added 3′ of the cDNA construct.