Figure 3. Histology of testis section from rescue animals compared to that of control heterozygous mice.

Paraffin embedded adult testis sections from transgenic lines eTg-F1 (a, b), eTg-F10 (c, d), pTg-M26 (e, f) and positive controls (g, h) were stained with haematoxylin for comparison of gross tissue architecture. The stained sections were viewed under 10× and 20× magnification using an Olympus 1×70 microscope. Note that both the size and overall architecture of seminiferous tubules of rescue mouse testes were restored in comparison to Ppp1cc −/− testis, and were comparable to those of positive control mice. Also notice that the seminiferous tubular lumens are replete with mature testicular spermatozoa, clearly seen in all rescue lines (white arrow) and in positive controls (black arrow). These observations are representative of multiple sections prepared from testes of several animals of each rescue and positive control line.