Table 1. Comparison of PPP1CC2 levels, testis weight, sperm number and morphology between transgenic rescue lines and control animals.

Transgenic lines	Normalized PPP1CC2 levels compared to Ppp1cc +/− level	Mean testis weight± SEM (in mg)	Mean Sperm number± SEM×107/ml	Sperm Morphology
				Total number of sperm counted	Normal (%)± SEM	Defective (%)± SEM
Ppp1cc +/−	n = 2, 1c	n = 10; 114.4±1.9c	n = 6; 3.9±0.3b	n = 1; 368	95	5c
Ppp1cc −/−	no expression	n = 6; 68.4±5.4a	–	–	–	–
eTg-F10	n = 2; 0.125a	n = 3; 110.5±1.9b,c	n = 3; 2.1±0.8a	n = 2; 323	32±2.8	68±2.8a,b
pTg-M3	n = 2; 0.125a	n = 2; 79.9±2.9a,b	n = 2; 1.4±0.05a	n = 2; 981	47±1.2	53±1.2a,b
eTg-M7	n = 2; 0.25a,b	n = 8; 107.4±4.6b,c	n = 5; 1.7±0. 9a	n = 2; 566	24±9.9	76±9.9a
eTg-F1	n = 5; 0.5a,b,c	n = 9; 102.7±2.9a,b	n = 5; 2.6±0.5a,b	n = 3; 1194	75±0.2	25±0.2a,b,c
pTg-M26	n = 3; 0.75b,c	n = 5; 108.2±4.2b,c	n = 4; 3.7±0.3a,b	n = 2; 986	88±2.3	12+2.3b,c
pTg-M30	n = 3; 1c	n = 4; 108.2±0.5b,c	n = 3; 3.7±0.5a,b	n = 2; 431	94±1.1	6±1.1c

The Kruskal-Wallis non-parametric one-way ANOVA by rank was performed and results were analyzed post-hoc by Dunn’s procedure for performing two-tailed multiple pair wise comparisons. Differences were considered significant if p<0.05 at a confidence interval of 95%.

a, b, c… denote significantly different groups.

SEM denotes Standard Error of the Mean.

n denotes number of samples/group.