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. 2012 Oct 17;7(10):e47896. doi: 10.1371/journal.pone.0047896

Figure 4. h2cR interacts specifically with the 5′UTR of hfq2 mRNA.

Figure 4

(A) EMSA experiments using 2.5 nM of the h2cR together with the indicated amounts of the hfq2 full transcript or (B) the hfq2 coding sequence; (C) EMSA experiments using with 2.5 nM of h2cR, together with the indicated amounts of the hfq2 5′UTR derivatives full 5′-UTR (nt −220 to +30), the RNA segment spanning nt −220 to −40 of the 5′-UTR, or the RNA segment spanning nt −70 to +30 of the 5′-UTR. For the full 5′-UTR and the RNA segment spanning nt −70 to +30 of the 5′-UTR 0, 0.1, 0.5, 2.5 or 10 nM of the respective 5′-UTR RNA derivatives were used, while for the RNA segment spanning nt −220 to −40 of the 5′-UTR 0, 0.1, 0,5, or 2.5 nM of RNA were used. (D) Ability of Hfq to bind 2.5 nM of h2cR (left panel) or the hfq2 5′-UTR (right panel), as evaluated by EMSA assays using the indicated concentrations of the hexameric form of Hfq. Apparent Kd values were calculated based on a semi-log plot of the RNA binding ratio versus protein concentration, using a exponential fit.