|
B. cenocepacia J2315 |
CF sputum isolate |
[44]
|
|
B. cenocepacia CJ1 |
B. cenocepacia J2315 derivative with the hfq gene interrupted by a Tp gene cassette |
[20]
|
|
B. cenocepacia CJ2 |
B. cenocepacia J2315 derivative with the hfq2 gene interrupted by a non-sense stop mutation |
[20]
|
|
B. cenocepacia J2315+ pSAS3 |
B. cenocepacia J2315 harbouring plasmid pSAS3 |
[20]
|
|
B. cenocepacia CJ1+ pSAS3 |
B. cenocepacia CJ1 mutant harbouring plasmid pSAS3 |
[20]
|
|
B. cenocepacia CJ4 |
B. cenocepacia J2315 harbouring plasmid pCGR17 |
This study |
|
B. cenocepacia CJ5 |
B. cenocepacia J2315 harbouring plasmid pCGR18 |
This study |
|
B. cenocepacia CJ6 |
B. cenocepacia CJ2 mutant harbouring plasmid pCGR27 |
This study |
|
B. cenocepacia CJ7 |
B. cenocepacia CJ4 harbouring plasmid pCGR27 |
This study |
|
B. cenocepacia CJ8 |
B. cenocepacia CJ5 harbouring plasmid pCGR27 |
This study |
|
B. cenocepacia CJ9 |
B. cenocepacia J2315 harbouring plasmid pCGR31 |
This study |
|
B. cenocepacia CJ10 |
B. cenocepacia J2315 harbouring plasmid pCGR32 |
This study |
|
B. cenocepacia CJ11 |
B. cenocepacia J2315 harbouring plasmid pMLBAD |
This study |
|
B. cenocepacia CJ12 |
B. cenocepacia J2315 harbouring plasmid pBBR1 |
This study |
|
E. coli BL21 |
Host for recombinant protein expression |
Stratagene |
|
E. coli TOP10 F' |
Host for recombinant plasmids |
Invitrogen |
| Plasmids |
|
|
| pCR II |
ApR; KmR; TOPO-TA cloning plasmid |
Invitrogen |
| pET23a+ |
ApR, used for C-terminal 6×histidine-tagged protein expression. |
Stratagene |
| pMLBAD |
TmpR; used for gene expression in Bcc |
[45]
|
| pBBR1MCS |
CmR; used for gene expression in Bcc |
[46]
|
| pSAS5 |
pWH884 with the hfq2 gene cloned |
[20]
|
| pSAS6 |
pET23a+ with the ompA gene clone |
(Sousa, SA, Leitão JH) Unpublished data |
| pSAS3 (phfq) |
pMLBAD with the hfq gene cloned |
[21]
|
| pCGR4 |
pET23a+ with the hfq gene |
[21]
|
| pCGR10 |
pCR II with the 136 bp cDNA fragment of the h2cR sRNA TA-cloned |
This study |
| pCGR11 |
pCR II with the 668 bp cDNA fragment of the hfq2 mRNA TA-cloned |
This study |
| pCGR12 |
pCR II with the 216 bp cDNA fragment of the 5′UTR hfq2 RNA TA-cloned |
This study |
| pCGR13 |
pCR II with the 216 bp cDNA fragment of the5′UTR hfq2 RNA cloned in the HindIII/BamHI sites (T7 promoter control) |
This study |
| pCGR14 |
pCR II with the 136 bp cDNA fragment of the h2cR RNA cloned in the HindIII/BamHI sites (T7 promoter control) |
This study |
| pCGR17 (ph2cR) |
pMLBAD with the 136 bp cDNA fragment of the h2cR RNA cloned in the XbaI/HindIII sites |
This study |
| pCGR18 (h2cRsil) |
pMLBAD with the 104 bp anti sense cDNA fragment of the h2cR with a cca extension cloned in the EcoRI/XbaI sites |
This study |
| pCGR26 |
pCGR13 with the his-tagged Hfq2 cloned in the HindIII/EcoRI sites |
This study |
| pCGR27 (pThfq2) |
pBBR1MCS with the N-terminus 6xhistidine tagged hfq2 and its complete 5′UTR cloned in the XhoI/HindIII sites |
This study |
| pCGR28 |
pCR II with the 180 bp cDNA fragment of the −220 to −40 region of hfq2 5′UTR cloned in the XbaI/HindIII sites |
This study |
| pCGR29 |
pCR II with the 100 bp cDNA fragment of the −70 to +30 region of hfq2 5′UTR cloned in the XhoI/BamHI sites |
This study |
| pCGR30 |
pCR II with the250 bp cDNA fragment of the −220 to +30 region of hfq2 5′UTR cloned in the XbaI/NotI sites |
This study |
| pCGR31 |
pBBR1 with the 136 bp cDNA fragment of the h2cR RNA cloned in the XbaI/HindIII sites |
This study |
| pCGR32 |
pBBR1 with the 104 bp anti sense cDNA fragment of the h2cR with a cca extension cloned in the EcoRI/XbaI sites |
This study |
