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. 2012 Oct 17;7(10):e47784. doi: 10.1371/journal.pone.0047784

Figure 5. Phosphorylation of 4E-BP1 in brains of PD subjects.

Figure 5

Western blot analysis of (A) frontal cortex or (B) basal ganglia soluble fractions from human control, idiopathic PD (iPD) and G2019S LRRK2 PD subjects with antibodies to total or phosphorylated (Thr37/46) 4E-BP1, or β-actin as a protein loading control. Molecular mass markers are indicated in kilodaltons (kDa). Densitometric analysis of 4E-BP1 phosphorylation (upper protein band) or total 4E-BP1 levels in idiopathic or G2019S PD brains compared to control brains. The levels of phosphorylated 4E-BP1 were normalized to total 4E-BP1, whereas total 4E-BP1 levels were normalized to β-actin levels, and expressed as a percent of control subjects (mean±SEM, n = 4–5 brains/group). For basal ganglia, G2019S subject 3 was excluded from the densitometric analysis due to a lack of detectable 4E-BP1 expression. *P<0.05 or **P<0.01 by one-way ANOVA with Newman-Keuls post-hoc analysis. ns, non-significant.