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. 2012 Oct 17;7(10):e44117. doi: 10.1371/journal.pone.0044117

Figure 3. Abnormal tau expression in ECM.

Figure 3

Aberrantly phosphorylated tau was evident in PbA infected mice when compared to control and PbN infected mice. (A) Brain lysates were probed with antibodies to PHF-1 which stains for phosphorylated tau at Ser396/404, and MC-1 which recognized misfolded tau protein. (B) PbA infected mice demonstrated a 48% higher in PHF-1 immunoreactivity compared to controls and a 134% more compared to PbN. Significant group effects on the means was demonstrated by one-way ANOVA (F(2, 8) = 14.85; p<0.01) with significant mean differences between PbA mice and both control and PbN mice using post-hoc Tukey's multiple comparison test. Tukey's test did not demonstrate a significant effect of PbN infection on the mean PHF-1 expression when compared to control mice. (C) PbA-infected mice displayed a 131% more MC-1 expression compared to controls and 137% higher expression compared to PbN-infected mice. One way ANOVA demonstrated significant group effects in the expression of MC-1 (F(2, 8) = 51.29; p<0.001) with post-hoc Tukey's test demonstrating significant effect of PbA infection on MC-1 expression when compared to either control or PbN mice, but no effect of PbN infection when compared to controls. Densitometry measurements are illustrated as a percentage of corresponding measurements in uninfected controls. Values plotted as mean ± SEM. *p<0.05, **p<0.001. Con = control, PbA = P. berghei ANKA infected mice, PbN = P. berghei NK65 infected mice. Ponceau was used as loading control.