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. 2012 Oct 17;7(10):e47742. doi: 10.1371/journal.pone.0047742

Figure 1. Characteristics of P. aeruginosa phage KPP12.

Figure 1

(A) Morphology. The bar indicates 100 nm. The head diameter and tail length were 62.5±2.5 nm and 124.2±3.4 nm (mean ± SD nm; n = 12), respectively. (B) Structural proteins. The structural proteins were separated using a 12.5% SDS-PAGE gel. The N-terminal of the structural proteins (indicated by arrows and numbers) were sequenced. The protein sequences (numbered from 1 to 3) were SNFTAPVTTPSIPTPIQFLQ, MFQKQVYRQYTPGFPGDLIE, and MINISAFGSIVQFTASRTFP, respectively. The proteins numbered from 1 to 3 were identified as ORF23, ORF22, and ORF30, respectively. (C) Phylogenetic analysis based on the major structural proteins of PB1-like phage. Phage KPP12 was closely related to phage LMA2 of the PB1-like viruses. (D) Stability. After iodixanol density gradient ultra-centrifugal purification, phage KPP12 was stored in the dark at 4°C for one month. Data are shown as mean values with SD (n = 3). The phage was fairly stable during the experimental period.