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. 2012 Oct 17;7(10):e47356. doi: 10.1371/journal.pone.0047356

Figure 5. Outside-in signaling mediated by αIIb in CHO cells expressing αIIbβ3.

Figure 5

(A) Spreading of CHO cells expressing αIIbβ3-WT, αIIbβ3-scramble and αIIbβ3-Δ724, respectively on immobilized Fg for 90minutes. (B) Quantification of area (pixel number) in 4 random fields (mean±SE) at all 6 time points. At the 90 minute time point, the size of CHO cells, CHO cells expressing αIIbβ3-WT, αIIbβ3-scramble and αIIbβ3-Δ724, was 24303.43 ± 4851.921 pixels, 97055.33 ± 21284.32 pixels, 133012.3 ± 71539.87 pixels, 24124 ± 11043.93 pixels, respectively. Statistical analysis performed using Student t test. (C) Akt Ser473 phosphorylation by CHO cells in suspension expressing αIIbβ3-WT, αIIbβ3-scramble and αIIbβ3-Δ724 induced by 5 µg/ml D3 and 100 µg/ml Fg. These experiments were repeated for three times.