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. 2012 Oct 17;7(10):e46222. doi: 10.1371/journal.pone.0046222

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© 2012 Jiménez et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Flow cytometry assay for the binding of the selected aptamer EJ4 with H23 (target cell line) and HBE135 E6/E7 (negative cell line). The green curve represents the background binding of a random sequence (library). Aim Figure 2: The aim of this figure is to show that the candidate aptamers are indeed aptamers because they do bind with the positive cell line (H23), but do not bind with the negative cell line (HBE135E6/E7).