Table 2. Binding of Adenocarcinoma aptamers with other cancer cell lines.

Cell line	Name	ADE1	ADE2	EJ2	EJ4	EJ5	EJ7
H23	Lung Adenocarcinoma	+++	+++	++++	++++	++	+++
HBE 135	Normal lung bronchial cell	_	_	_	_	_	_
A549	Lung Adenocarcinoma	++	_	_	++	_	_
H460	Large cell carcinoma	_	_	++	++	_	_
H520	Squamous cell carcinoma	_	_	_	_	_	_
CAOV3	Ovary adenocarcinoma	_	_	_	_	_	_
TOV21G	Ovary clear cell carcinoma	++	_	++	++	_	_
DLD1	Colon adenocarcinoma	_	_	+++	_	_	_

A threshold based on the fluorescence intensity of FITC in the flow cytometric analysis was chosen so that 99% of cells incubated with the FITC-labeled unselected DNA library would have lower fluorescence intensity than the threshold. When the FITC-labeled aptamer was allowed to interact with the cells, the percentage of the cells with fluorescence above the set threshold was used to evaluate the binding capacity of the aptamer to the cells. (−) 0<10%, 10–35% +, 35–60% ++, 60–85% +++, >85% ++++.