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. Author manuscript; available in PMC: 2013 Oct 3.
Published in final edited form as: Cell Metab. 2012 Oct 3;16(4):462–472. doi: 10.1016/j.cmet.2012.08.011

Figure 5. siRNA-knockdown of Cullin-3 increases RhoA in rat aortic smooth muscle cells.

Figure 5

(A–B) 1° rat aortic SMC treated with negative contr ol siRNA (NC1) or two different siRNAs targeting Cullin-3 were assessed for protein levels of Cullin-3 and total Nedd8-conjugates (Nedd8) (A) and Cullin-3 substrates RhoA, RhoBTB1, and Cyclin E (B) by Western blot (n=6). U, untreated; nc1, negative control siRNA; si1, Cullin-3 siRNA #1; si2, Cullin-3 siRNA #2. * p<0.05 Cullin-3 siRNA-treated vs. NC1 siRNA-treated. Error bars represent SEM.