Fig. 4. Loss of β-catenin through the osterix-cre causes increased adipogenic and reduced osteogenic gene expression.

(A–C) qRT-PCR analysis of mRNAs encoding wnt target genes (A), adipogenic genes (B) and osteogenic transcripts (C) in BMSCs isolated from 8-wk old tibiae and femurs of β-cat^f/f (CON) and osx-cre;β-cat^f/f (KO) mice treated with Dox until sacrifice, and cultured under either adipogenic or osteogenic condition for 14 or 21 days, as indicated. Data are plotted as mean ± SEM. *p < 0.05 vs CON-derived cells cultured for the same time. Results were obtained from four independent experiments (4 mice per genotype).